| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01085
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-222
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-03027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-03038
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-01012
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01054
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2021-03-12^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f9ef1f934ed4d16480068e9c27daece
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_160556fb9d09e2a54ffadbfa38c712c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3283cd811cb537ddccec23a451a6c562 |
| publicationDate |
2021-07-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113151129-A |
| titleOfInvention |
Construction method of recombinant Escherichia coli with high yield of p-aminophenylalanine |
| abstract |
The invention relates to and discloses a method for constructing recombinant Escherichia coli with high yield of p-aminophenylalanine. The present invention screened the PAPA biosynthesis pathway from different strains, and determined the pathway source of high-yielding PAPA through combinatorial optimization. The combined pathway was transferred into Escherichia coli, and the host strain was genetically engineered to strengthen the chorismate synthesis pathway and weaken its metabolic pathway. Recombinant Escherichia coli that can finally produce high PAPA is obtained. The PAPA yield of this strain can reach 752.63mg/L. The invention lays a foundation for the industrialized production and application of PAPA. |
| priorityDate |
2021-03-12^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |