http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113249357-B

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filingDate 2021-06-22^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-09-14^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-09-14^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113249357-B
titleOfInvention Rhamnosidase TpeRhha-H570A mutant and preparation method and application thereof
abstract The invention discloses a rhamnosidase TpeRhha-H570A mutant and a preparation method and application thereof, relates to the technical field of biological medicine, and aims to provide a mutant with an amino acid sequence shown as SEQ ID NO: 2 to alanine at position 570 of the TpeRha enzyme. The rhamnosidase TpeRhha-H570A mutant shows high specificity in the application of catalyzing and hydrolyzing icariin to generate icariside I, and the product conversion rate is improved by 15.63 times compared with the wild TpeRhha enzyme. The rhamnosidase TpeRhha-H570A mutant can be applied to biological processing to prepare epimedium extracts with different component contents, and has short enzymolysis time and high conversion rate.
priorityDate 2021-06-22^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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