http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113358797-A

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publicationDate 2021-09-07^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113358797-A
titleOfInvention A chromatographic analysis method for simultaneous analysis of flavonoids, phenylpropane, coumarin and phenolamide secondary metabolites in fresh tobacco leaves
abstract The invention discloses a chromatographic analysis method for simultaneously analyzing flavonoids, phenylpropane, coumarin and phenolamide secondary metabolites in fresh tobacco leaves, comprising the following steps: A. Microwave-assisted extraction of secondary metabolites in fresh tobacco leaves; B. The extract is analyzed by ultra-high performance liquid chromatography-ultraviolet detector-tandem quadrupole-time-of-flight mass spectrometry; C. Secondary metabolites are identified, and the identified 13 kinds of flavonoids, 19 kinds of phenylpropane, Relative quantitative analysis of 4 coumarins and 6 phenolamides. A microwave-assisted extraction-ultra-high performance liquid chromatography-ultraviolet detector-tandem quadrupole-time-of-flight mass spectrometry established by the present invention simultaneously measures 13 kinds of flavonoids, 19 kinds of phenylpropane, 4 kinds of coumarins and 6 kinds of coumarins in fresh tobacco leaves This method is a kind of phenolamide-based method, which has the characteristics of various and comprehensive qualitative and quantitative metabolites, and can be used for rapid, high-sensitivity and stable high-throughput analysis of flavonoids, phenylpropanes, coumarins and phenolamides in fresh tobacco leaves. biological metabolites.
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