| abstract |
Diverse cDNA libraries derived from cell-free mRNA and methods for making the same are provided. The library can be prepared by extracting RNA from body fluids such as serum or plasma, separating the RNA from contaminants, synthesizing cDNA using reverse transcriptase, and enriching for protein-encoding nucleotide sequences. The library can include a variety of transcripts from solid tissues. Cf-RNA can be measured by qPCR, sequencing, or other suitable methods. |