| abstract |
The invention discloses a miRNA analog related to the ovarian development of Portunus trituratus and its application. The miRNA analog is one or more of let-7-5p and miR-141-3p. In the present invention, after the synthesis of let-7-5p agomir and miR-141 agomir, the two are simultaneously injected into female crabs through the base of the third step, so as to down-regulate the key enzyme genes of the methylfarnesate decomposition pathway JHE-like CXE-1 and JHE-like CXE- 2 are expressed and the expression of vitellogenin gene vtg is up-regulated, so as to achieve the purpose of promoting exogenous yolk synthesis of Portunus trituratus, and accelerating the development and maturation of the ovary of Portunus trituratus, and the method of the present invention and The prepared accelerator has little side effect on the parent body, is efficient and reliable, and is suitable for promoting the ripening of a large number of parent bodies. |