http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114561444-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3ae70b461288533d6451ad98ff81518 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9126 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2022-03-22^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75a68d894a9de880447e6522078bc2fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53472a201ab4ef70870afed45bd930fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a3ec4137c40be8557edeb6a7af3601 |
| publicationDate | 2022-05-31^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114561444-A |
| titleOfInvention | A method for the detection of Bst DNA polymerase activity |
| abstract | The present invention provides a method for detecting the activity of Bst DNA polymerase. Using double-stranded circular plasmid DNA as a template, in the presence of dNTP mix and two pairs of isothermal amplification primers, Bst DNA polymerase is used for isothermal amplification to prepare Bst The standard curve of DNA polymerase standard substance and pyrophosphate content, the activity of Bst DNA polymerase is calculated according to the standard curve by detecting the pyrophosphate content in the amplified product of the sample to be tested. The activity of Bst DNA polymerase is proportional to the amount of double-stranded DNA product within a certain range, and the amount of DNA product is proportional to the pyrophosphate in the reaction system, so its activity can be measured by the fluorescence intensity of the pyrophosphate kit. characteristics, developed the method of the present invention. The invention utilizes the high polymerizability and strand displacement activity of Bst DNA polymerase to rapidly measure the polymerization activity of Bst DNA polymerase by detecting the fluorescent signal of the amplified product, has no radioactive pollution, and has the advantages of simplicity, rapidity, high sensitivity and the like. |
| priorityDate | 2022-03-22^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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