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filingDate 2022-06-13^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_066dc2465f275555203398794152df92
publicationDate 2022-07-15^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114752677-A
titleOfInvention A primer set, kit, amplification method and application for rapid detection of IDH1 mutation
abstract The present application relates to the field of gene detection, in particular to a primer set, kit, amplification method and application for rapid detection of IDH1 mutation; the primer set includes IDH1 R132H gene mutant probe, IDH1 R132L gene mutant probe, IDH1 R132C gene mutant probe, IDH1 R132G gene mutant probe and IDH1 R132S gene mutant probe; the kit includes a primer set, and the amplification method includes: extracting an isolated glioma tissue sample; The glioma tissue sample is pretreated to obtain the sample slurry containing the target gene; the sample slurry and the magnetic bead solution are added to the kit, and then the PCR amplification reaction is performed to obtain a purified amplification product; the application includes: primers The kit is used in the preparation of detection reagents for clinical detection of IDH1 mutation sites; all mutation types can be detected after one round of detection in the same kit.
priorityDate 2022-06-13^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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