http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114773446-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_774bcf26b1bad9ec25ecafc6e82028db |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43572 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 2022-06-16^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ec8a3f475ed545a11d0f12e4a1ecd01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ff018ebadac4f98ae828ca94501ca38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af082e8c7fe67f6f1e600c3a074d3f0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2398c5f69c5dd14d025c343b860dd017 |
| publicationDate | 2022-07-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114773446-A |
| titleOfInvention | A kind of melittin and its separation and purification method |
| abstract | The invention discloses a melittin and a separation and purification method thereof, and relates to the field of biotechnology. The method includes: performing the first reverse-phase C18 semi-preparative column separation on the crude extract of bee venom to be separated and purified, using formic acid aqueous solution and acetonitrile as mobile phases for gradient elution, collecting the eluent, and then lyophilizing the eluent After redissolving, the reconstituted solution is used for secondary reversed-phase C18 semi-preparative column separation, and the secondary reversed-phase C18 semi-preparative column separation is isocratic elution using trifluoroacetic acid aqueous solution and methanol as mobile phases. The method can achieve the technical effects of high separation purity, less loss of target substances, and less amount of organic solvent without the need to combine ultrafiltration process to remove impurities in a specific pH environment, and has the advantages of environmental friendliness, simple device, simple process operation and suitable for industrial production. Technical advantages. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115197304-A |
| priorityDate | 2022-06-16^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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