http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114891060-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dfe1de44c89046277ed8a139812b6f55 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2570-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 |
| filingDate | 2022-07-13^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4eb5c02e3298884daca3db1a741bb1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d912038c46815136172eb9f2e1df29d6 |
| publicationDate | 2022-08-12^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114891060-A |
| titleOfInvention | A light-activation-dependent proximity labeling method for proteins and their applications |
| abstract | The invention belongs to the field of biotechnology, and in particular relates to a light-activated-dependent proximity labeling method for proteins, and its application in preparing a kit for studying protein-protein interactions. The method includes: fusion expression of a target protein and a photosensitive protein to obtain a first system containing the fusion protein; chemical probes containing amino groups and alkyne groups are provided, the first system and the chemical probes are mixed and incubated for a preset time and then illuminated with visible light processing to obtain the second system; providing a click chemistry reaction reagent containing biotin-labeled azide, mixing the second system with the click chemistry reagent and performing the click chemistry reaction, and then performing proteomic analysis to obtain the interaction with the target protein information on neighboring proteins. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115494243-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115494243-B |
| priorityDate | 2022-07-13^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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