http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0019937-A1

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filingDate 1980-06-03^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92babf5c18eebe393da568e54a2f46cd
publicationDate 1980-12-10^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0019937-A1
titleOfInvention A method for producing alcohol oxidase, alcohol oxidase, enzyme preparation and a method for determining the concentration of a compound in an alcohol-containing sample
abstract A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.
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