| abstract |
A method for identifying and quantifying allergen specific IgE levels in patient serum by conjugating the IgE in the serum with allergens adhering to an insoluble support, conjugating the serum IgE with an enzyme labeled anti-lgE antibody, contacting the enzyme label with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluoresence in the solution. Special reagents and their manufacture are also disclosed. |