| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_09d637fa076e23c43de51a8685e2bc73 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1014
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6481 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-22 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate |
1985-08-27^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83aceb7cc1b1f0918f7dd0d2672781a2 |
| publicationDate |
1986-04-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0178764-A1 |
| titleOfInvention |
Method of stabilizing a host microorganism/expression vector system for large scale production of proteins |
| abstract |
This invention provides a method of stabilizing a host microorganism / expression vector system for large scale production of a protein which comprises transforming an auxotrophic strain of said microorganism, having at least one mutation in the tryptophan operon such that the cells are unable to produce sufficient tryptophan to survive or grow, with a recombinant vector which comprises (a) a gene which encodes a protein of interest and is under the control of regulatory sequences for expression and (b) one or more functional trp genes, under the control of regulatory sequences for expression, which at least complement the mutation(s) in the tryptophan operon of the host cells thereby enabling the said host cells to synthesize tryptophan. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0543344-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0405858-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6057146-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5891710-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6291245-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5824496-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0972838-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0405858-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0251579-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0251579-A3 |
| priorityDate |
1984-08-28^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |