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filingDate 1990-12-03^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1991-06-05^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0430719-A2
titleOfInvention Compounds and reagents for determination of reticulocytes
abstract Quaternized derivatives of acridine orange are used for quantitatively determining reticulocyte levels in whole blood by fluorescence flow cytometry techniques. The quaternized derivatives of acridine orange are of the general formula:n n wherein X is a benzyl group of formulan n wherein R₁ is hydrogen or fluorine, and R2 is fluorine, trifluoromethyl or hydrogen; or X may be a hydroxyethylene group; and γ⁻ is bromide or iodide.
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