| abstract |
Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigenes. Particularly, the β2-microglobulin gene is inactivated for reducing or eliminating Class I MHC antigenes. The resulting cells may be used as universal donors. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts. |