| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2817954cd008bcd609a4c4eb49050be |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00659
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00637
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00612
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00585
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00596
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00527
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00707
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00387
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0059 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate |
1999-04-30^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d8f5b5e9bf6f6bd6e38ce5c26fd34bb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_765b3ffdc11f70fc33d01d7f6c737f09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35fb1528870f8cbacffab313608b3f75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d276e7ffc5d3ef81a4e6d24541cb3e59 |
| publicationDate |
2001-02-07^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1073770-A2 |
| titleOfInvention |
Novel method for the identification of clones conferring a desired biological property from an expression library |
| abstract |
The present invention relates to a novel method for the identification and/or characterization of clones conferring a desired biological property from an expression library. The method of the invention comprises the step of analyzing for the expression of at least one (poly)peptide, such as a tag expressed as a fusion protein, together with a recombinant insert of a clone of said expression library, wherein the clones of said expression library are arranged in arrayed form. Said (poly)peptide may be fused N-terminally or C-terminally to said insert. The method of the invention further comprises the steps of contacting a ligand specifically interacting with a (poly)peptide expressed by the insert of a clone conferring said desired biological property with a first replica of said library of clones in arrayed form and analyzing said library of clones for the occurrence of an interaction, and/or carrying out a hybridization or an oligonucleotide fingerprint with a nucleic acid probe specific for the insert of a clone conferring said desired biological property with a second replica of said library of clones arranged in arrayed form and analyzing said library of clones for the occurrence of a specific hybridization. Finally, the method of the invention requires the identification of clones wherein an expression of the at least one (poly)peptide in step (a) and/or an interaction in step (b) and/or a hybridization or an oligonucleotide fingerprint in step (c) can be detected. The present invention also relates to a kit useful for carrying out the method of the invention. |
| priorityDate |
1998-04-30^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |