| abstract |
The present invention relates to a process for secretory production of a foreignnprotein, in particular, transglutaminase by a coryneform bacterium. n According to the present invention, a process is provided for the secretorynproduction of a foreign protein, in particular, transglutaminase, by making ancoryneform bacterium to produce an industrially useful foreign protein, in particular,ntransglutaminase and efficiently release the product extracellularly (i.e., secretorynproduction). n An intended foreign protein, in particular, transglutaminase, is produced bynusing an expression construct wherein the gene sequence of the intended foreignnprotein containing the pro-structure part, in particular, pro-transglutaminase genensequence, is ligated to the downstream of a sequence encoding the signal peptidenregion from a coryneform bacterium, introducing this expressional genetic constructninto a coryneform bacterium, culturing the thus transformed coryneform bacterium,nand treating the extracellularly released protein with a protease, etc. to cleave andneliminate the pro-part. |