| abstract |
It is an object of the present invention to providenisozymes of the lachrymatory factor producing enzyme, thenamino acid sequences of these isozymes and a gene that codesnfor these amino acid sequences, and the present inventionnrelates to three types of isozymes of the lachrymatory factornproducing enzyme that contributes to the production of thenlachrymatory factor that is present in onions and the like,namino acid sequences indicated by SEQ ID Nos. 1 to 3 whichnconstitute the proteins or polypeptides of these isozymes, DNAnindicated by SEQ ID Nos. 4 and 5 which contains base sequencesnthat code for the abovementioned proteins or polypeptides, anmethod of producing the abovementioned isozymes, a recombinantnvector which contains the abovementioned DNA, a transformantnformed by transforming a host cell with the abovementionednrecombinant vector, a method of producing proteins ornpolypeptides that have lachrymatory factor producing enzymenactivity by culturing the abovementioned host cell, and anti-sensenRNA which has a base sequence that is complementary tonthat of the mRNA corresponding to the abovementioned DNA. |