http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1436391-A4

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_414229135a401ef1f2bb85926092929f
classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-113
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
filingDate 2002-06-05^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26b551e2ce73f1b3124eb9356f471791
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb1cbee2a971b05a880a19d8cc657647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf5a8ca47d989ec56c4fb901d28a076f
publicationDate 2005-02-16^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1436391-A4
titleOfInvention CYCLIC EXTENSION AT LOW TEMPERATURE OF DNA WITH HIGH SPECIFICITY OF BEGINNING
abstract The invention relates to methods for extending a primer or a pair of primers in low-temperature cycle DNA amplification for cycle sequencing and PCR. In particular, the methods contemplate the combined use of moderately thermostable DNA polymerases in the presence of a low concentration of glycerol or ethylene glycol, or the mixtures thereof, as an agent to reduce the melting temperature of DNA (that is, the temperature at which the double-strands of DNA are denatured). Predistributed reaction mixtures of a high-fidelity and high processivity DNA polymerase stable at room temperature for several weeks in ready-to-use kits are also contemplated by the invention.
priorityDate 2001-06-08^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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