| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2003-09-04^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-02-29^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6c89f66200a808303dfdda68716e248
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_821c84a515f8292033b4c2d97406a970
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0e089379f6b882f59b0b18bedbfc813 |
| publicationDate |
2012-02-29^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1546405-B1 |
| titleOfInvention |
Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase |
| abstract |
The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry. |
| priorityDate |
2002-09-13^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |