http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2256195-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T428-2991 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2009-06-03^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26fe431424ca921f6e2da7cbec835ca8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66d8fbeb11c18f075c1893fd1f98eabb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c65f40622a9d1d424e2956969fdda5b |
| publicationDate | 2010-12-01^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2256195-A1 |
| titleOfInvention | Nucleic acid purification method |
| abstract | The present invention relates to a process for the purification of a defined amount of nucleic acids from a nucleic acid-containing sample, which comprises at least the following steps: a. Contacting the nucleic acid-containing sample with a defined amount of a nucleic acid-binding phase having the following characteristics: (i) the nucleic acid binding phase has nucleic acid binding ligands having at least one protonatable group; (ii) the nucleic acid binding ligands are attached to a support; (iii) the nucleic acid binding phase has a low charge density surface, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid-binding phase used; b. Binding the nucleic acids to the nucleic acid binding phase at a pH (binding pH) which is below the pKa of at least one of the protonatable groups; c. Elution of the nucleic acids at a pH above the binding pH, whereby a defined amount of nucleic acids is obtained.n n n In addition, corresponding kits as well as nucleic acid-binding phases which can be used for the purification of nucleic acids are disclosed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3180432-A4 |
| priorityDate | 2009-05-12^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Showing number of triples: 1 to 71 of 71.