http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2336354-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-10^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa6f2ce5e3b6bd7221adf1d49b7b7f28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d120dbeb1c42f4c70bb2510142a4b029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a6f7392022b07d9df491f695d63e1fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d761f638f284a3d388942aa4610f9c7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_246cf1ddf8f7c1ee43d596c13cf0054b |
| publicationDate | 2011-06-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2336354-A1 |
| titleOfInvention | A method for the detection of a RNA molecule, a kit and a use related thereof |
| abstract | In a first aspect, the present invention relates to a method for the detection of a RNA molecule, said method comprising the steps of providing a sample containing the RNA molecule; hybridizing to said RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. n In a further aspect, the present invention relates to a kit comprising a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety. n In another aspect, the present invention relates to the use of said kit for the detection of an RNA molecule. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2706124-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3225721-A4 |
| priorityDate | 2009-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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