| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_68470742c37db68ce35b8ae425ce50cc |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-916
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate |
2012-01-31^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ab91826ad2776ca667e9b123b0d8be1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60d77eb17a4fe4475432594d794acd82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52ed2cb3ec32a57912e9b9fde3e45ebc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_504c716600f16ed12f69500cba50f1fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_676c26ba2791bf644d9efd4540be883c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f597dedafcc5092610018f5a08039789
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21c94812fa199b1817b99c7853420b62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_754cae4f28cd84a48de9561bbe80287d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_516c66e1a084fe28f9334f31974f7f9e |
| publicationDate |
2014-12-10^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2810064-A1 |
| titleOfInvention |
Cell based sensor |
| abstract |
Cell based sensor. The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a non-protease hydrolase as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis. Said granule stored non protease-hydrolase reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific substrate; absence of toxicity when cell thawing; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection. |
| priorityDate |
2012-01-31^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |