http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2935622-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6846 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-12-17^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11441dfa4a974e174464714e09e37411 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78d6167fc0bb04a3cd5b6c695b9bb6a0 |
| publicationDate | 2015-10-28^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2935622-A1 |
| titleOfInvention | Nucleic acid amplification method |
| abstract | The invention relates to a method of performing a helicase dependent amplification (HDA) or thermophilic helicase dependent amplification of a template nucleic acid comprising: (i) combining in a reaction mixture the template nucleic acid; a forward and a reverse HDA primer; a helicase; at least one DNA polymerase and deoxynucleotide triphosphates (dNTPs), (ii) wherein the reaction comprises a linear polyethylene glycol with the formula (I) wherein n is between 2 to 50, preferably n is selected from the group comprising 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12. The invention also relates to a kit comprising one or more reagents for performing an HDA reaction, wherein a polyethylene glycol is present in the kit. |
| priorityDate | 2012-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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