| abstract |
The present invention relates to a method for the in vitro preparation of stem cells from ruminant somatic cells by the exogenous expression of the CDX-2 and c-MYC genes in said somatic cells. The invention also relates to the stem cells thus obtained, as well as their use for the expansion of an individual genetic background, due to the amplification of the initial somatic cell gene pool, for the screening of anti-proliferative molecules and the test the action of anti-proliferative molecules, and to replicate and test the virulence of certain pathogens. These stem cells exhibit certain characteristics of pluripotent stem cells, such as self-renewal, infinite proliferation, detection of telomerase activity, specific cell cycle, and expression of particular markers. |