| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1bc9c4662bc64b2a6a52e6da1ecd908e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 |
| filingDate |
2017-01-16^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b790e0c5bea2e8db1ac454efcad9f0b5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_637e0b3d6800ef399d0dadb6ac6b6b2d |
| publicationDate |
2018-11-21^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-3402886-A1 |
| titleOfInvention |
Method of producing a production cell line |
| abstract |
A method for producing a eukaryotic production cell line expressing a protein of interest (POI), comprising a) incorporating a gene of interest (GOI) encoding said POI into the chromosome of a eukaryotic host cell within an exogenous euchromatin protein expression locus by transfection, thereby obtaining a repertoire of recombinant host cells in a pool; b) selecting a single cell from said pool within 12 days after transfection, wherein selecting is at least according to the expression of said GOI or a marker indicating said expression; and c) isolating and expanding the selected single cell, thereby obtaining the production cell line. |
| priorityDate |
2016-01-14^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |