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publicationDate 2019-10-02^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-3546484-A1
titleOfInvention In vitro production of red blood cells with sortaggable proteins
abstract A method for conjugating a peptide of interest to the surface of red blood cells, the method comprising:providing a genetically engineered red blood cell presenting a first fusion protein comprising a first red blood cell membrane protein and a first peptide, and contacting the red blood cell with a first peptide of interest in the presence of a first sortase under conditions suitable for the sortase to conjugate the first peptide of interest to the first peptide in the fusion protein,wherein either the first peptide in the fusion protein or the first peptide of interest comprises a first sortase recognition motif.
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