http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2426289-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ffc50cc1a8b55071f575b2020093c21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca50b55e204e2809f135b32717c64e6a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-22^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d90ba9441c087565ad1e9b287485aa0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cefc0823f0485ebf566491e0b2065125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89baf1b0853973d1595b4556ad62963c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32ce57cde4b156099e8c8b332531c3d3 |
| publicationDate | 2013-10-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2426289-T3 |
| titleOfInvention | Staphylococcus aureus detection and identification of methicillin-resistant Staphylococcus aureus |
| abstract | A specific detection procedure for the presence of a Staphylococcus aureus (S. aureus) strain and specific detection and identification of a methicillin-resistant S. aureus strain of a clinical sample in a single assay, comprising contacting said sample with at least one primer and / or nuc probe of at least 13 nucleotides that is inhibited under stringent conditions with a specific sequence for S. aureus within SEQ ID NO: 200 or the complement thereof; contacting said sample with at least one MREJ primer and / or probe of at least 13 nucleotides that is inhibited under stringent conditions with polymorphic MREJ nucleic acids within at least one of the following SEQ ID NOs specific for MREJ: 14, 15, 16 , 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31,32, 33, 34, 35, 36, 37, 38, 39, 40, 41 , 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66 , 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87 or 88, or the complement thereof , wherein the at least one primer and / or probe hybridize together under stringent conditions with specific orfX sequences for S. aureus species and polymorphic sequences of the right limb of SCCmec; and detect the presence and / or quantity of hybridized nuc and MREJ probe (s) as indicative of the presence and / or amount of S. aureus and MRSA in the sample, respectively, or detect the amount of an amplification product produced by hybridization of the primers nuc and MREJ with nucleic acids, as an indication of the presence and / or quantity of S. aureus and MRSA, respectively, in which said stringent conditions comprise 4 mM MgCl2, 100 mM Tris (pH 8.3), 10 mM KCl , (NH4) 2SO4 5mM, 0.15 mg / ml BSA, 4% trehalose at 59 ° C. |
| priorityDate | 2006-12-19^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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