| abstract |
Method for preparing a polypeptide comprising: (a) providing an amino acid sequence of an original polypeptide having asparaginase activity; (b) select at least one amino acid residue at a position in the sequence that corresponds to any of positions 54, 57, 70, 83, 84, 86, 102, 137, 164, 196, 201, 228, 260, 262 , 278, 283, 290, 307, 312, 323, 327, 334, 336, 337, 349, 351, 353, 366 and / or 375 in SEQ ID NO: 1; (c) modify the sequence by substitution of the selected amino acid residue; (d) produce a polypeptide variant having the modified sequence; (e) test the polypeptide variant for asparaginase activity and thermotolerance; and (f) selecting a polypeptide variant with asparaginase activity and higher thermotolerance compared to the original polypeptide. |