http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2546186-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d22b649c54d79d7c1eabf23085b14a3e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S424-831 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-807 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-6037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-627 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-646 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-6415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-48 |
| filingDate | 2008-01-02^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-21^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e998765461840c59a581a9e6cb4ada7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1b7c94d7aba0e5817d6da830ad9f54b |
| publicationDate | 2015-09-21^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2546186-T3 |
| titleOfInvention | Vaccine Manufacturing Procedure |
| abstract | A method of manufacturing an immunogenic composition comprising a conjugation step of conjugation of a saccharide to a protein vehicle to produce a protein-saccharide conjugate using carbodiimide condensation chemistry, in which the saccharide comprises (for example, as part of its repeating unit) or has been derivatized to comprise, amino and / or carboxyl groups, and in which the protein carrier comprises, or has been derivatized to comprise, amino and / or carboxyl groups, which comprises the steps of I) - if the protein vehicle comprises both amino and carboxyl groups and the saccharide comprises amino or carboxyl groups: a) mixing the saccharide and aliquot of carbodiimide necessary to carry out the conjugation, and b) adding the aliquot of the necessary protein vehicle for a period from 1 minute to 6 hours, in which at least a quarter of the aliquot is added in the first half of the period and at least a quarter of the aliquot in the second half of the period; II) - if the saccharide comprises both amino and carboxyl groups and the protein vehicle comprises amino or carboxyl groups: a) mixing the protein vehicle and the aliquot of carbodiimide necessary to carry out the conjugation, and b) adding the saccharide aliquot necessary for during a period of 1 minute to 6 hours, in which at least a quarter of the aliquot is added during the first half of the period, and at least a quarter of the aliquot in the second half of the period; III) - if the saccharide comprises both amino and carboxyl groups and the protein vehicle comprises both amino and carboxyl groups: a) mixing the protein carrier and the saccharide; and b) add the aliquot of carbodiimide necessary to perform the conjugation for a period of 1 minute to 6 hours, in which at least a quarter of the aliquot is added during the first half of the period and at least a quarter of the aliquot during the second half of the period; and in which: the aliquot of carbodiimide is 0.01 to 3 mg of carbodiimide / mg of saccharide; the saccharide is present at a final concentration of 0.5-50 mg / ml in step b); the protein vehicle is present at a final concentration of 1-50 mg / ml in step b); The initial ratio between the protein vehicle and the saccharide is 5: 1 to 1: 5 (w / w); the salt concentration present in step b) is 0-2M; the pH of the reaction in step b) is pH 4.5-6.5, pH 4.5-7.5 or if a compound is present in step b) that keeps the reaction intermediate stable; and the reaction temperature in step b) is 4-37 degrees C, and an additional step of mixing the protein-saccharide conjugate with a staphylococcal antigen. |
| priorityDate | 2007-01-04^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Showing number of triples: 1 to 482 of 482.