| abstract |
A chromatographic separation process to recover a polyunsaturated fatty acid (PUFA) product from a feed mixture, comprising: (a) purifying the feed mixture in a first chromatographic separation stage using as eluent a mixture of water and a first organic solvent, obtaining an intermediate product; and (b) purifying the intermediate product in a second chromatographic separation stage using as eluent a mixture of water and a second organic solvent, obtaining the AGPI product, the second organic solvent being different from the first organic solvent, and in which the The first chromatographic separation stage comprises introducing the feed mixture into a simulated or real mobile bed chromatography apparatus, and the second chromatographic separation stage comprises introducing the intermediate product into a stationary bed chromatography apparatus. |