http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2772259-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84e7b6ec9b1b3c07d91fdba21afe8453 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C2601-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C2602-24 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07J9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C401-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07J9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C401-00 |
| filingDate | 2015-01-14^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-07^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2b5f33a55e96c7790763e22769d689e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa64f2b2a1a457eec21c3cdc2ef0604a |
| publicationDate | 2020-07-07^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2772259-T3 |
| titleOfInvention | Manufacturing method of 7-dehydrocholesterol and vitamin D3 |
| abstract | A process for producing 7-dehydrocholesterol, 7DHC, comprising: cultivating, in medium, a Labyrinthulea microorganism that produces 7DHC in which the reducing activity of 7DHC is reduced or lost compared to a parent strain by deletion, substitution, or addition of at least one base in a gene that is present in the chromosomal DNA of the parent strain and encodes a protein that exhibits 7DHC reducing activity and the microorganism produces 7DHC; allow 7DHC to be produced and accumulated in the culture; and collecting the 7DHC from the culture, wherein the gene encoding the protein exhibiting 7DHC reducing activity is a gene exhibiting any of the following DNAs [1] to [6]: [1] a DNA encoding a protein having the amino acid sequence represented by SEQ ID No. 2; [2] a DNA encoding a mutated protein consisting of the amino acid sequence SEQ ID No. 2 by deletion, substitution or addition of 1 to 20 amino acids and exhibiting a 7DHC reducing activity; [3] a DNA encoding a homologous protein exhibiting at least 95% identity with the amino acid sequence represented by SEQ ID NO: 2 and exhibiting 7DHC reducing activity; [4] a DNA having the base sequence represented by SEQ ID No. 1; [5] a DNA that hybridizes with DNA that consists of a base sequence complementary to the base sequence represented by SEQ ID No. 1 under stringent conditions and encodes a homologous protein that exhibits a 7DHC reducing activity; and [6] a DNA exhibiting at least 95% identity with the base sequence represented by SEQ ID NO: 1 and encoding a homologous protein exhibiting 7DHC reducing activity. |
| priorityDate | 2014-01-17^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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