| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abcd8589ad479b9f9835c57e3aa535c0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-151
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-119 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate |
2017-08-16^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-03-23^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e9eed789311a11a7a0db660f4c206b0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6124ad0bc897c0a706730bfa959a3867
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13b67feb28039eab882313c896ff7840 |
| publicationDate |
2021-03-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2813324-T3 |
| titleOfInvention |
Production of closed linear DNA |
| abstract |
A cell-free method for producing closed linear deoxyribose nucleic acid (DNA) molecules comprising: (a) contacting a template comprising a linear double-stranded DNA molecule covalently closed at each end by a portion of a sequence of protelomerase recognition and comprising at least one stem-loop motif with a strand displacement polymerase under conditions that promote amplification of said template in the presence of at least one primer that is capable of specifically binding to a primer binding site within said loop stem motif; (b) contacting the DNA produced in (a) with at least one protelomerase under conditions that promote the production of closed linear DNA. |
| priorityDate |
2016-08-16^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |