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filingDate 1973-05-30^^<http://www.w3.org/2001/XMLSchema#date>
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publicationNumber GB-1439358-A
titleOfInvention Electric circuits
abstract 1439358 Centrifugal analyzer; determination of glucose UNION CARBIDE CORP 30 May 1973 [31 May 1972] 25805/73 Heading G1B [Also in Division G4] A centrifugal analyzer comprises a rotory PTFE disc having cavities 3, 5 into which reagents are placed and chambers 302, into which the reagents are forced on rotation, connected by passages 306 with cuvettes 9. The course of the reaction is followed by measurement with a photo-multiple 13 of light from lamp 11 transmitted through the curvettes. The determination of glucose in blood serum in the above apparatus is described using a reagent containing NADP (nicotineamide adernine dinucleotide phosphate), hexokinase, glucose-6- phosphate dehydrogenase and MgSO 4 in a triethonolamine buffer at pH 7À5. The resulting reduction of NADP is followed at a wavelength of 340 nm.
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