| abstract |
PROBLEM TO BE SOLVED: To immobilize a DNA fragment prepared in advance separately on a solid-phase carrier surface by a rapid reaction, and a reaction product can stably maintain a bond. Develop a method. SOLUTION: A DNA fragment is brought into contact with the surface of a fixed layer of a solid support having a fixed layer in which inorganic fine particles are dispersed in a cured film of a water-soluble resin cross-linked by a cross-linking agent in a liquid phase. Thus, a method for immobilizing a DNA fragment on the surface of the immobilization layer at one end thereof, the method for immobilizing a DNA fragment on the surface of a solid support, the DNA chip obtained by this method, DN using DNA chip A method for detecting a nucleic acid fragment having complementarity to a DNA fragment on the A chip. |