| abstract |
A genetically engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing over 25% eicosapentaenoic acid (EPA, ω-3 polyunsaturated fatty acid) in the total oil fraction is described. These strains comprise various chimeric genes that express heterologous desaturases, elongases, and acyltransferases, and optionally comprise various natural desaturases and acyltransferase knockouts, enabling EPA synthesis and high accumulation To. A production host cell is claimed and a method for producing EPA in said host cell is also claimed. |