| abstract |
The present invention relates to a method for producing a desired protein (eg, a desired heterologous protein) comprising the steps of: (a) a first recombination encoding a protein comprising the sequence of a first chaperone protein. A host comprising a gene, a second recombinant gene encoding a protein comprising a sequence of a second chaperone protein, and a third gene encoding a desired protein (eg, a desired heterologous protein), eg, a recombinant gene Prepare cells, where the first and second chaperones are different, and (b) cultivate the host cells in medium to express the first, second and third genes. |