| abstract |
PROBLEM TO BE SOLVED: To evaluate at least one of a viral protein of a virus to be captured and a viral nucleic acid thereof, and an arrival position of a permeation target protein in a polymer film at the same time in a polymer film, and To provide a virus separation membrane design method based on this evaluation method. A method for evaluating a fine particle arrival position in a polymer film including the following steps (a) to (h) in order. (A) A solution containing a virus and a permeation target protein is sent from the primary side of the membrane to the secondary side, (B) taking a cross section of the membrane, (C) Multiple staining of two or more kinds of fine particles present in one section with different fluorescent dyes, (D) Two or more kinds of fine particles are observed with a fluorescence microscope according to each dye, (E) Each cross-section to be observed is set as image data, and subdivided into equally divided planes parallel to the filtration direction. (F) Quantifying the fluorescence signal of each equally divided surface for each dye, (G) integrating each histogram for each dye, (H) The arrival position of the fine particles is determined. [Selection figure] None |