http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011125337-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-16^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6af9e8a0c0368188c9de60e7775abd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6b6b69465eafc646409c340f16c54fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e05595b694aae86673e17170b789e378 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b50ed846d6796a155555f4115d68c613 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea7ad53945811b6e17aa8706c7b91cf0 |
| publicationDate | 2011-06-30^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2011125337-A |
| titleOfInvention | RNA molecule detection method, kit and use thereof |
| abstract | An improved method for detecting low RNA molecules is provided. A) providing a sample containing an RNA molecule; b) hybridizing a first polynucleotide to the RNA molecule; c) reverse-trancribing the RNA molecule to generate first strand cDNA; d ) Hybridizing the second polynucleotide to the first strand cDNA, the second polynucleotide comprising: (i) a 3′-part complementary to a portion of the first strand cDNA (ii) second primer binding A 3′-nonextendible oligonucleotide comprising a 5′-overhang comprising the sequence of the site, e) obtaining an extension reaction product comprising a sequence complementary to the second primer binding site from the first strand cDNA, f A) amplifying the extension reaction product by polymerase chain reaction using a first primer and a second primer complementary to the second primer binding site; g) detecting the amplification product by real-time fluorescence readout; Of RNA molecules, including Way out. [Selection figure] None |
| priorityDate | 2009-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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