| abstract |
The present disclosure characterizes therapeutic antibodies (eg, TPO mimetic antibodies) and therapeutically active fragments thereof and methods of preparing and using the antibodies and fragments. For example, the therapeutic antibodies and fragments thereof are useful in a variety of diagnostic and / or therapeutic applications, such as methods of increasing platelet levels in a subject. In one aspect, a therapeutic antibody or a therapeutically active fragment of the antibody is provided, the antibody or the fragment comprising at least two therapeutic peptides, wherein at least one of the therapeutic peptides is the therapeutic Is incorporated into the constant region of the light chain polypeptide at a position that structurally allows the peptide to be present in the central cleft of the antibody. |