http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013040800-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f017de30242de1c99ace84ba61e5d5d4 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate | 2011-08-11^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_957b0d18723b0b6fe3b3c92cb77377e8 |
publicationDate | 2013-02-28^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2013040800-A |
titleOfInvention | Cell release material detection device, cell release material detection method, and immobilized enzyme substrate for cell release material detection |
abstract | [Object] To provide a cell-releasing substance detection device, a cell-releasing substance detection method, and an immobilized enzyme substrate for detecting a cell-releasing substance for detecting a concentration distribution on a cell tissue of nucleotide-related chemical substances released from cells. Cell tissue S is placed on a transparent substrate 10 on which GAPDH is immobilized, and ATP on the surface of cell tissue S is consumed in the presence of GAP and NAD + to produce NADH. The evanescent wave L2 is generated on the surface of the transparent substrate 10 by the irradiation light from the light source unit 7 provided on the side of the transparent substrate 10, and fluorescence of NADH in the immediate vicinity of the transparent substrate 10, that is, the surface of the cell tissue S is emitted. Let By designing the reaction system in this way, ATP, which is a ribonucleotide, can be detected by the fluorescence emitted by the fluorescent substance (NADH) generated by the reaction. [Selection] Figure 1 |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021241446-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017101957-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020020896-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015114630-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102409313-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7226651-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20220040667-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2021241446-A1 |
priorityDate | 2011-08-11^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
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