http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015513405-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2549-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-03-11^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-05-14^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2015513405-A |
| titleOfInvention | Composition for hot start reverse transcription reaction or hot start reverse transcriptase chain reaction |
| abstract | The present invention relates to a composition for hot start reverse transcription reaction and a composition for reverse transcription PCR containing the same, and more specifically, a reaction buffer solution, MgCl 2 , four kinds of dNTPs, and reverse transcription polymerization in one reaction tube. The present invention relates to a reaction composition obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing an enzyme, and a hot start reverse transcription reaction composition having improved stability and long-term storage by freezing or drying the reaction mixture. The present invention relates to a composition for sequentially performing a hot start reverse transcription reaction and a PCR reaction in one reaction tube by additionally adding a polymerizing enzyme, and a nucleic acid amplification method using the composition. The hot start reverse transcription reaction composition prevents non-specific reverse transcription reaction in the normal temperature mixing process compared with the existing reverse transcription reaction composition, and selectively reverse transcription with respect to the target RNA at the reaction temperature. Since the reaction can be performed and the PCR can proceed and the RNA amplification reaction can be carried out with high sensitivity, it can be used easily and usefully for multiplex reverse transcription PCR or real-time quantitative reverse transcription PCR method. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019129818-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7234598-B2 |
| priorityDate | 2012-03-09^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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