| abstract |
PROBLEM TO BE SOLVED: To provide a method for 21-hydroxylation of a steroid in a cell. A method of hydroxylating a carbon atom 21 of a steroid. a) (i) heterologous CYP21A2 protein or functional variant thereof, (ii) at least one heterologous electron transport chain capable of transmitting electrons to CYP21A2, and (iii) one or more that promotes folding of CYP21A2. Steps to prepare cells expressing the chaperone of b) The method comprising adding a steroid to the cells. [Selection diagram] None |