| abstract |
(57) [Summary] [Purpose] To easily amplify target nucleic acid. [Structure] Cyclic single-stranded nucleotide (A), primer (B) having a sequence at least partially complementary to said nucleotide (A) or fragmented target nucleic acid sequence (D) And a primer (B) annealed to a circular single-stranded nucleotide (A) using a thermostable polymerase (C) Alternatively, the fragmented target nucleic acid sequence (D) is extended to generate a single-stranded nucleic acid sequence having a sequence in which a sequence complementary to the cyclic single-stranded nucleotide (A) is repeated. |