http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0698799-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1993-08-03^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ca5d5e5f32d558effcc9f454d16a604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a3a06c3206ff0bb94506ab12baf6fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c266b77b2fb7af2b9a8e2089eaa86080 |
| publicationDate | 1994-04-12^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H0698799-A |
| titleOfInvention | Polynucleotide detection method |
| abstract | (57) [Summary] [Object] An object of the present invention is to provide a method for detecting a polynucleotide suitable for a highly sensitive, highly accurate, quantitative, and high throughput automated apparatus. [Constitution] A fluorescent labeled probe 2 which hybridizes to a target polynucleotide chain 1 is used as a fluorescent labeled probe, and both are reacted to obtain a fluorescent labeled probe 3 bound to the target polynucleotide 1. After unreacted excess fluorescently labeled probe was separated and removed by electrophoresis, the temperature was raised to above the denaturation temperature of the conjugate 3 of the target polynucleotide and the fluorescently labeled probe, and then electrophoresis was continued to bind to the target polynucleotide. The fluorescently labeled probe 4 is released, and the released fluorescently labeled probe 4 is irradiated with the laser light beam 6, and the emitted fluorescence is detected by the fluorescence detector 7. [Effect] There is an advantage that a series of steps can be sequentially and automatically performed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7135331-B2 |
| priorityDate | 1992-08-06^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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