http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11127893-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f62824da9d41b863ecaed02e6fd87d2 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate | 1997-10-28^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5822925c9f02865a1abc549154fdbacd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_217adfdc1dc0ef31b5c640636c3cfcf6 |
publicationDate | 1999-05-18^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-H11127893-A |
titleOfInvention | Enzymatic degradation of AMP |
abstract | An object of the present invention is to provide a method for quantifying a substrate or an enzyme using an ADP-dependent hexokinase (ADP-HK) reaction, in which adenylate kinase (MK) coexisting in a sample is used. It is an object of the present invention to provide a method for improving the quantitativeness by avoiding the influence of the above on the reaction system. SOLUTION: AM generated in an ADP-HK reaction system Hydrolyzing P by an enzyme, in particular AMP deaminase, AMP nucleosidase or 5 ′ nucleotidase, A method for substantially removing AMP from the reaction system. Said A ADP of MK coexisting in sample by MP removal method A method for substantially eliminating the effect on the HK reaction system, and a method for hydrolyzing the AMP, wherein ADP-HK is allowed to act on the sample to measure the enzyme activity and / or substrate concentration in the sample. Methods and reagents therefor. |
priorityDate | 1997-10-28^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
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