http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H11506333-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 1996-06-03^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1999-06-08^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H11506333-A |
| titleOfInvention | Enzyme catalysis in organic solutions |
| abstract | (57) [Summary]nThe organic enzyme solution contains an enzyme and a surfactant ion-pair complex, and an organic solvent in which the enzyme-surfactant ion pair is dissolved, and the solution contains equal amounts of the enzyme and the ion-pair complex. It has at least an order of magnitude higher catalytic activity than suspensions of organic solvents that do not contain it. The enzyme is preferably a hydrolase having acyltransferase activity, and the surfactant is a low concentration of Aerosol OT (sodium bis (2-ethylhexyl) sulfosuccinate). The organic enzyme solution is obtained by obtaining an aqueous solution of the enzyme having a pH of the maximum enzyme activity, adding an ionic surfactant to the aqueous solution in an amount sufficient to form an ion pair, and adding an enzyme-surfactant ion pair. By extracting into the organic solvent phase without substantial formation of reverse micelles to produce an anhydrous organic enzyme solution containing enzyme-surfactant ion pairs dissolved in the organic solvent (so that the enzyme has the natural structure And retain substantial catalytic activity). The catalyst solution according to the present invention can be used to catalyze peptide synthesis from acyl donors and nucleophiles. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009028034-A |
| priorityDate | 1995-06-01^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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