http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63304986-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f8cfa5aee4410611b5c562275c9f8e3 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
filingDate | 1987-06-04^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e4a3d36b911cc64a503760f3eeff9ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2fb8b4df9b1c684c983b3264ad252c96 |
publicationDate | 1988-12-13^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S63304986-A |
titleOfInvention | Plasmid |
abstract | PURPOSE:To obtain a novel plasmid containing a gene to code AlaDH, AlaR and D-ATA derived from a thermostable bacterium useful for enzymatic synthesis of D-amino acid. CONSTITUTION:DNA fragment containing a gene to code AlaDH (alanine dehydrogenase) and AlaR (alanine racemase) derived from chromosome DNA of Bacillus stearothermophilus IFO12550 and DNA fragment containing a gene to code D-ATA (D-amino acid transaminase) derived from chromosome DNA of Bacillus sp. YM-1 (FERM P-8057) are prepared. The fragments and vector plasmid pUC18 are digested with ECoRI or SacI and linked with ligase T4 to constitute the aimed plasmid PICRT11. The plasmid is transduced to Escherichia coli HB101, etc., and a transformant is cultivated in a medium to produce AlaDH, AlaR and D-ATA. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1818411-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2202317-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2202318-A1 |
priorityDate | 1987-06-04^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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