| abstract |
An object of the present invention is to develop a method for detecting GPCR (G protein-coupled receptor) signaling by measuring ligand stimulation-dependent cAMP production using a germinating baculovirus. According to the present invention, a host infected with at least one recombinant baculovirus containing a gene encoding a G protein-coupled receptor protein, a gene encoding a G protein, and a gene encoding adenylate cyclase is cultured. Recovering the germinated baculovirus released from the host, contacting the germinated baculovirus with a ligand, and assaying the produced cAMP, to detect G protein-coupled receptor signaling. Provided. |