http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-0166424-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_52a24331fe7882d8a0801155df05d542 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 1995-07-01^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1999-02-01^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2664d0127d2f1445397ed381fac272fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d25183bf9664618d660890e31f82d50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3904b34dcab3824291a7c4e603987d72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bd1da5eb24342bf5d75fe874dd1f7ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb394cb93a9e9dceca1b4dafa11c82a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86390d2474c3e9674c52b468009601ea |
| publicationDate | 1999-02-01^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-0166424-B1 |
| titleOfInvention | Method for preparing target protein from fusion protein using urokinase |
| abstract | The present invention expresses a specific amino acid sequence cleaved by urokinase between the target protein of the fusion protein and the fusion partner, and cleaves the expressed fusion protein with urokinase, thereby cutting the target protein from the fusion protein. It relates to a manufacturing method. In the present invention, when the amino acid sequence of the urokinase cleavage site located between the target protein and the fusion partner of the fusion protein is -X-Gly-Arg (X is Pro, Thr, Ile, Phe or Leu), the cleavage efficiency is excellent. It was also found that the expression of the fusion protein including the cleavage site was excellent, and it was confirmed that by using the amino acid sequence of the urokinase cleavage site determined in the present invention, it was possible to economically and easily manufacture a large amount of the protein from the fusion protein. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100859088-B1 |
| priorityDate | 1995-07-01^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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