http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101064783-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2005-11-04^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-09-14^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-09-14^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101064783-B1 |
| titleOfInvention | How to Promote Single Protein Production in Living Cells Using MRNA Interferase |
| abstract | The present invention discloses an SPP system in living E. coli cells utilizing the unique properties of mRNA interferases, wherein said mRNA interferases are single-stranded RNA- and ACA-specific endoribonu as bacterial toxins. Clease MazF can be exemplified, which MazF efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a sharp decrease in total protein synthesis. Concomitant expression of MazF with target genes engineered to encode ACA-deficient mRNA results in sustained target protein expression in the absence of background cell protein synthesis, resulting in high levels of target up to 90%. Induces protein synthesis Surprisingly, the synthesis of target proteins lasts at least 4 days, suggesting that the cells retain transcriptional and translational capacity despite their growth retardation. SPP technology is well suited for yeast and human proteins, and even for bacterial endogenous membrane proteins. The new system can greatly simplify the structural and functional studies of proteins that were previously considered biologically important but difficult to handle due to their unparalleled signal-to-noise ratio. n n mRNA interferase, SPP system, MazF |
| priorityDate | 2004-11-04^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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